6MFX
Crystal structure of a 4-domain construct of a mutant of LgrA in the substrate donation state
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CLSI BEAMLINE 08ID-1 |
| Synchrotron site | CLSI |
| Beamline | 08ID-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-10-02 |
| Detector | DECTRIS PILATUS3 S 6M |
| Wavelength(s) | 0.979 |
| Spacegroup name | P 1 |
| Unit cell lengths | 67.449, 73.987, 77.076 |
| Unit cell angles | 94.31, 114.86, 92.23 |
Refinement procedure
| Resolution | 69.580 - 2.200 |
| R-factor | 0.1963 |
| Rwork | 0.195 |
| R-free | 0.24400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6mfw |
| Data reduction software | DIALS |
| Data scaling software | DIALS |
| Phasing software | PHASER |
| Refinement software | PHENIX (dev_3584) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 69.580 | 2.250 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Number of reflections | 66903 | 4469 |
| <I/σ(I)> | 9.1 | 1.6 |
| Completeness [%] | 98.0 | 96.7 |
| Redundancy | 3.3 | |
| CC(1/2) | 0.991 | 0.388 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.1 | 295 | 26% PEG4000, 0.1 M Tris-HCl, pH 8.1 |
