6MFN
Human Argonaute2-miR-27a bound to HSUR1 target RNA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 23-ID-D |
| Synchrotron site | APS |
| Beamline | 23-ID-D |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-11-21 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 1.033230 |
| Spacegroup name | P 2 21 21 |
| Unit cell lengths | 66.948, 104.212, 152.858 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 28.590 - 2.500 |
| R-factor | 0.2109 |
| Rwork | 0.209 |
| R-free | 0.24680 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4w5o |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.706 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 28.590 | 2.600 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.061 | 0.516 |
| Rpim | 0.039 | 0.323 |
| Number of reflections | 37628 | 4159 |
| <I/σ(I)> | 14.5 | 2.3 |
| Completeness [%] | 99.3 | 98.5 |
| Redundancy | 3.3 | 3.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 293 | 10% PEG3350, 50 mM Tris, pH 8.0, 20 mM magnesium chloride, 75 mM phenol |
