6MEI
Crystal structure of broadly neutralizing antibody HEPC3 in complex with Hepatitis C virus envelope glycoprotein E2 ectodomain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 23-ID-D |
| Synchrotron site | APS |
| Beamline | 23-ID-D |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-10-28 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 65.810, 75.790, 173.999 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 86.999 - 2.900 |
| R-factor | 0.2112 |
| Rwork | 0.208 |
| R-free | 0.27690 |
| Data reduction software | MOSFLM |
| Data scaling software | Aimless (0.5.32) |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.13_2998) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 87.000 | 87.000 | 2.270 |
| High resolution limit [Å] | 2.200 | 9.070 | 2.200 |
| Rmerge | 0.271 | 0.092 | 0.663 |
| Rmeas | 0.310 | 0.105 | 0.819 |
| Rpim | 0.145 | 0.049 | 0.467 |
| Total number of observations | 231097 | 3599 | 15221 |
| Number of reflections | 44996 | 761 | 3808 |
| <I/σ(I)> | 3.7 | 8.3 | 0.3 |
| Completeness [%] | 99.8 | 100 | 98.7 |
| Redundancy | 5.1 | 4.7 | 4 |
| CC(1/2) | 0.885 | 0.976 | 0.660 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 298 | 0.2M sodium bromide, 20% PEG 3,350 |
