6MDZ

Human Argonaute2-miR-122 bound to a target RNA with two central mismatches (bu2)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL12-2
Synchrotron site	SSRL
Beamline	BL12-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-03-03
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.979500
Spacegroup name	P 21 21 21
Unit cell lengths	107.615, 138.289, 152.610
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	38.293 - 3.400
R-factor	0.2219
Rwork	0.220
R-free	0.26430
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4w5o
RMSD bond length	0.002
RMSD bond angle	0.571
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	39.500	3.580
High resolution limit [Å]	3.400	3.400
Rmerge	0.166	0.564
Rpim	0.080	0.268
Number of reflections	31940	4579
<I/σ(I)>	8.4	3
Completeness [%]	99.5	99
Redundancy	5.3	5.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8	293	10% PEG3350, 50mM Tris pH8, 20mM MgCl2, 75mM Phenol