6MDT
Crystal structure of the B41 SOSIP.664 Env trimer with PGT124 and 35O22 Fabs, in P63 space group
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL12-2 |
Synchrotron site | SSRL |
Beamline | BL12-2 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2017-02-22 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.97946 |
Spacegroup name | P 63 |
Unit cell lengths | 129.343, 129.343, 313.071 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 41.956 - 3.816 |
R-factor | 0.2952 |
Rwork | 0.294 |
R-free | 0.31110 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4r26 5cez |
RMSD bond length | 0.004 |
RMSD bond angle | 0.911 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | PHASER |
Refinement software | PHENIX ((1.12_2829)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 3.940 |
High resolution limit [Å] | 3.800 | 3.800 |
Number of reflections | 28747 | 2842 |
<I/σ(I)> | 6.4 | 1.8 |
Completeness [%] | 99.9 | 99.9 |
Redundancy | 10.9 | 9.6 |
CC(1/2) | 0.770 | 0.270 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 277.15 | 0.1M Calcium acetate, 0.1M MES (pH 6), 15%(v/v) PEG400 |