6MD0
Crystal Structure of Human PPARgamma Ligand Binding Domain in Complex with Oleic Acid
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 |
| Temperature [K] | 200 |
| Detector technology | IMAGE PLATE |
| Collection date | 2018-02-22 |
| Detector | MAR scanner 345 mm plate |
| Wavelength(s) | 1.5418 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 92.930, 62.170, 119.320 |
| Unit cell angles | 90.00, 102.20, 90.00 |
Refinement procedure
| Resolution | 38.880 - 1.950 |
| R-factor | 0.225 |
| Rwork | 0.223 |
| R-free | 0.27300 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2vv2 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.11.1_2575) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 38.880 | 2.020 |
| High resolution limit [Å] | 1.950 | 1.950 |
| Rmerge | 0.036 | 0.471 |
| Rmeas | 0.051 | 0.666 |
| Rpim | 0.036 | 0.471 |
| Number of reflections | 46477 | |
| <I/σ(I)> | 8.16 | 1.34 |
| Completeness [%] | 95.3 | 93.96 |
| Redundancy | 1.7 | 1.6 |
| CC(1/2) | 0.785 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.4 | 293 | 0.1M MOPS, pH 7.4 0.8M Sodium Citrate |
