6MCY

Crystal structure of mouse Bak

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Synchrotron site	Australian Synchrotron
Beamline	MX2
Temperature [K]	100
Detector technology	CCD
Collection date	2015-03-11
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.9537
Spacegroup name	P 1
Unit cell lengths	36.951, 59.261, 79.207
Unit cell angles	93.14, 93.35, 97.63

Refinement procedure

Resolution	36.543 - 1.748
R-factor	0.182
Rwork	0.181
R-free	0.21780
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2imt
RMSD bond length	0.006
RMSD bond angle	0.750
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX ((1.13_2998: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	36.540	1.811
High resolution limit [Å]	1.748	1.748
Rmerge	0.110
Rpim	0.065
Number of reflections	65510	6395
<I/σ(I)>	9.08	0.72
Completeness [%]	97.5	95.24
Redundancy	3.9
CC(1/2)	0.997	0.326

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		281	20% PEG 3350, 200 mM ammonium formate