6MC9

Crystal Structure of Human Nav1.4 C-Terminal (1599-1754) domain in complex with calcium-bound calmodulin

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS-II BEAMLINE 17-ID-2
Synchrotron site	NSLS-II
Beamline	17-ID-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-06-23
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.979
Spacegroup name	P 43 21 2
Unit cell lengths	72.615, 72.615, 134.661
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	40.829 - 3.300
R-factor	0.2421
Rwork	0.240
R-free	0.28530
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3g43
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.13_2998)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	40.829	40.829	3.380
High resolution limit [Å]	3.300	14.750	3.300
Rmerge	0.119	0.048	1.841
Rmeas	0.121	0.049	1.879
Total number of observations	145128
Number of reflections	5845	83	406
<I/σ(I)>	18.11	46.54	2.08
Completeness [%]	99.9	96.5	100
Redundancy	24.829	16.831	24.865
CC(1/2)	0.999	0.997	0.683

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	6	293	0.1M MES pH 6.0, 20% PEG 6000, 1.0M LiCl