6M7K
Structure of mouse RECON (AKR1C13) in complex with cyclic AMP-AMP-GMP (cAAG)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-C |
| Synchrotron site | APS |
| Beamline | 24-ID-C |
| Temperature [K] | 80 |
| Detector technology | PIXEL |
| Collection date | 2018-08-16 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 0.97910 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 50.597, 57.070, 110.760 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 46.020 - 1.100 |
| R-factor | 0.143 |
| Rwork | 0.143 |
| R-free | 0.15740 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5uxf |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.421 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.13_2998: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.020 | 1.120 |
| High resolution limit [Å] | 1.100 | 1.100 |
| Rpim | 0.029 | |
| Number of reflections | 130194 | 6242 |
| <I/σ(I)> | 14 | 2.6 |
| Completeness [%] | 99.7 | 97.5 |
| Redundancy | 9.1 | 8.2 |
| CC(1/2) | 0.999 | 0.813 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 291 | 1.0 M lithium chloride, 0.1 M sodium acetate, 30% PEG6000 |
