6M6M

The crystal structure of glycosidase mutant

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U
Synchrotron site	SSRF
Beamline	BL17U
Temperature [K]	100
Detector technology	CCD
Collection date	2018-10-28
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.9791
Spacegroup name	P 1 21 1
Unit cell lengths	52.734, 95.432, 93.382
Unit cell angles	90.00, 90.76, 90.00

Refinement procedure

Resolution	32.634 - 2.070
R-factor	0.1354
Rwork	0.134
R-free	0.17820
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3ta9
RMSD bond length	0.007
RMSD bond angle	0.875
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((1.10.1_2155: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.110
High resolution limit [Å]	2.070	2.070
Number of reflections	342172	342172
<I/σ(I)>	27.6
Completeness [%]	99.9
Redundancy	3.7
CC(1/2)	0.990	0.990

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.5	293	0.1 M Tris-HCl (pH 8.5), 0.2 M Magnesium chloride, PEG 8000 (20%, w/v)