6M1U

Crystal structure of the vertebrate conserved region (VCR) of human METTL16

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-17A
Synchrotron site	Photon Factory
Beamline	BL-17A
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-06-11
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.98
Spacegroup name	H 3 2
Unit cell lengths	139.610, 139.610, 53.130
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	48.641 - 2.791
R-factor	0.2349
Rwork	0.234
R-free	0.26300
Structure solution method	SAD
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.13_2998)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	48.641	48.641	2.860
High resolution limit [Å]	2.790	12.480	2.790
Rmerge	0.343	0.065	4.761
Rmeas	0.346	0.065	4.791
Total number of observations	410237
Number of reflections	5022	65	356
<I/σ(I)>	17.68	66.17	1.26
Completeness [%]	99.8	97	98.1
Redundancy	81.688	64.892	81.792
CC(1/2)	0.999	0.999	0.634

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	100 mM Tris-HCl, pH 7.4, 20% (w/v) PEG3350, 200 mM sodium citrate, 4% (w/v) formamide and 5 mM dextran sulfate sodium salt.