6M0V

Crsytal structure of streptococcus thermophilus Cas9 in complex with the GGAA PAM

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	200
Detector technology	PIXEL
Collection date	2019-02-05
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.978
Spacegroup name	C 1 2 1
Unit cell lengths	321.839, 75.078, 70.109
Unit cell angles	90.00, 93.21, 90.00

Refinement procedure

Resolution	48.822 - 3.000
R-factor	0.2156
Rwork	0.213
R-free	0.25660
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5axw
RMSD bond length	0.003
RMSD bond angle	0.647
Data reduction software	HKL-3000
Data scaling software	HKL-2000
Phasing software	HKL-3000
Refinement software	PHENIX (1.10.1_2155)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	3.110
High resolution limit [Å]	3.000	3.000
Number of reflections	31477	1824
<I/σ(I)>	11.6
Completeness [%]	99.8
Redundancy	6.6
CC(1/2)	0.800	0.800

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		290	KCL, MgCl2, MPD, MgCl2