6LZC
crystal structure of Human Methionine aminopeptidase (HsMetAP1b) in complex with KV-P2-4H-05
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ELETTRA BEAMLINE 11.2C |
Synchrotron site | ELETTRA |
Beamline | 11.2C |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2019-05-28 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 0.95 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 47.552, 77.219, 48.651 |
Unit cell angles | 90.00, 91.55, 90.00 |
Refinement procedure
Resolution | 47.530 - 1.350 |
R-factor | 0.1689 |
Rwork | 0.167 |
R-free | 0.19720 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2g3h |
RMSD bond length | 0.017 |
RMSD bond angle | 2.293 |
Data reduction software | XDS |
Data scaling software | Aimless (0.7.4) |
Phasing software | MOLREP (11.7.01) |
Refinement software | REFMAC (5.8.0238) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 47.580 | 47.530 | 1.380 |
High resolution limit [Å] | 1.350 | 7.410 | 1.350 |
Rmerge | 0.042 | 0.028 | 0.722 |
Rmeas | 0.046 | 0.031 | 0.785 |
Rpim | 0.017 | 0.012 | 0.302 |
Total number of observations | 3194 | 24093 | |
Number of reflections | 76213 | 495 | 3718 |
<I/σ(I)> | 20.1 | 57.9 | 2.5 |
Completeness [%] | 99.7 | 99.3 | 98.5 |
Redundancy | 6.8 | 6.5 | 6.5 |
CC(1/2) | 0.999 | 0.999 | 0.907 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.2 | 298 | 0.1M Bistris pH-6.2, 19% PEG 3350, 5% Glycerol |