6LZ9

t8E4 antibody Fab complexed with the active form of HGF

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL41XU
Synchrotron site	SPring-8
Beamline	BL41XU
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-10-27
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	1.0
Spacegroup name	P 63 2 2
Unit cell lengths	176.180, 176.180, 122.960
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	47.870 - 2.800
R-factor	0.2506
Rwork	0.248
R-free	0.29750
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1shy 1opg
RMSD bond length	0.007
RMSD bond angle	0.971
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX (1.17.1_3660)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.970
High resolution limit [Å]	2.800	2.800
Number of reflections	28218	4412
<I/σ(I)>	13.8
Completeness [%]	99.7
Redundancy	9.9
CC(1/2)	0.998	0.673

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	293	10 % (w/v) PEG 10000, 0.1M Magnesium acetate, 0.1M MES pH 6.5