6LYL

Crystal structure of S1052D mutant of Formylglycinamidine synthetase

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	Cu FINE FOCUS
Temperature [K]	120
Detector technology	IMAGE PLATE
Collection date	2016-11-30
Detector	RIGAKU RAXIS IV++
Wavelength(s)	1.5417
Spacegroup name	P 65
Unit cell lengths	146.193, 146.193, 141.053
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	47.850 - 2.100
R-factor	0.1546
Rwork	0.153
R-free	0.19190
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1t3t
RMSD bond length	0.018
RMSD bond angle	1.927
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	REFMAC (5.8.0135)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.140
High resolution limit [Å]	2.100	2.100
Rmeas	0.107
Rpim		0.267
Number of reflections	99120	4898
<I/σ(I)>	15.9
Completeness [%]	99.5
Redundancy	5.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		298	2M Ammonium Sulphate