6LY7
PylRS C-terminus domain mutant bound with 1-Formyl-L-tryptophan and AMPNP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSRRC BEAMLINE BL15A1 |
| Synchrotron site | NSRRC |
| Beamline | BL15A1 |
| Temperature [K] | 110 |
| Detector technology | CCD |
| Collection date | 2017-03-27 |
| Detector | RAYONIX MX300HE |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 64 |
| Unit cell lengths | 105.346, 105.346, 72.396 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 28.355 - 2.094 |
| R-factor | 0.173943199964 |
| Rwork | 0.173 |
| R-free | 0.18385 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2zce |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.103 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.11.1_2575) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 28.355 | 2.180 |
| High resolution limit [Å] | 2.090 | 2.090 |
| Rmeas | 0.104 | 0.829 |
| Rpim | 0.039 | 0.308 |
| Number of reflections | 26941 | 1908 |
| <I/σ(I)> | 33.2 | 2.5 |
| Completeness [%] | 96.9 | |
| Redundancy | 7.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | 100 mM HEPES, 10% PEG 8000, 10% Ethylene Glycerol |
