6LUE
Crystal structure of mouse Cryptochrome 1 in complex with compound KL201
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL44XU |
| Synchrotron site | SPring-8 |
| Beamline | BL44XU |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2017-06-30 |
| Detector | Bruker DIP-6040 |
| Wavelength(s) | 0.9 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 130.551, 143.260, 76.730 |
| Unit cell angles | 90.00, 118.65, 90.00 |
Refinement procedure
| Resolution | 40.460 - 2.100 |
| R-factor | 0.2203 |
| Rwork | 0.218 |
| R-free | 0.25560 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4k0r |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.661 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 41.450 | 2.175 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.133 | 0.450 |
| Rpim | 0.091 | 0.310 |
| Number of reflections | 71867 | 10493 |
| <I/σ(I)> | 6.6 | 2.8 |
| Completeness [%] | 99.9 | |
| Redundancy | 3.1 | |
| CC(1/2) | 0.982 | 0.545 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | 20% w/v PEG 3350, 3% v/v ethylene glycol, 200 mM NH4Cl |
