6LSH

Crystal structure of the enterovirus 71 polymerase elongation complex (C2S6M form)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U
Synchrotron site	SSRF
Beamline	BL17U
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-12-08
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.9792
Spacegroup name	P 21 21 21
Unit cell lengths	62.878, 77.380, 155.770
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	28.900 - 2.231
R-factor	0.2098
Rwork	0.208
R-free	0.24450
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5f8l
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (v1.14)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.310
High resolution limit [Å]	2.230	4.800	2.230
Rmerge	0.045	0.028	0.503
Rmeas	0.049	0.031	0.576
Rpim	0.021	0.013	0.272
Total number of observations	196215
Number of reflections	35885	3952	2929
<I/σ(I)>	13
Completeness [%]	95.2	98.4	79.1
Redundancy	5.5	5.4	3.9
CC(1/2)		0.999	0.843

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	6.5	289	MES, PEG 5000 monomethyl ether, glycerol