6LSF

Crystal structure of the enterovirus 71 polymerase elongation complex (C2S6RA/C2S6RB form)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U
Synchrotron site	SSRF
Beamline	BL17U
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-11-07
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.9792
Spacegroup name	P 21 21 21
Unit cell lengths	61.350, 76.447, 155.004
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	28.729 - 2.152
R-factor	0.2169
Rwork	0.215
R-free	0.25060
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5f8l
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.14)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.230
High resolution limit [Å]	2.150	4.630	2.150
Rmerge	0.067	0.049	0.408
Rmeas	0.076	0.055	0.466
Rpim	0.034	0.025	0.220
Total number of observations	170510
Number of reflections	36176	3962	2655
<I/σ(I)>	12.1
Completeness [%]	89.5	92.1	66.8
Redundancy	4.7	4.7	3.7
CC(1/2)		0.995	0.878

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	6.5	289	MES, PEG 5000 monomethyl ether, glycerol