6LRA
The complex structure of PHF core domain peptide of tau and antibody's Fab domain.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | Cu FINE FOCUS |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2017-07-13 |
| Detector | RIGAKU RAXIS VII |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 59.650, 70.680, 118.370 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.420 - 1.900 |
| R-factor | 0.19149 |
| Rwork | 0.188 |
| R-free | 0.24892 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2qhr 2gsg |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.678 |
| Data reduction software | CrystalClear |
| Data scaling software | CrystalClear |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0258) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 60.685 | 1.970 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.070 | 0.303 |
| Number of reflections | 39804 | 3848 |
| <I/σ(I)> | 14.9 | |
| Completeness [%] | 99.0 | |
| Redundancy | 6.86 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 23% PEG3350, 20% glycerol, 0.2 M NH4I, 10 mM Tris-HCl pH 8.0 |
