6LPF
The crystal structure of human cytoplasmic LRS
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL19U1 |
Synchrotron site | SSRF |
Beamline | BL19U1 |
Temperature [K] | 97 |
Detector technology | PIXEL |
Collection date | 2017-05-26 |
Detector | DECTRIS PILATUS3 S 6M |
Wavelength(s) | 0.97775 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 88.217, 94.684, 680.055 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 49.077 - 2.490 |
R-factor | 0.2007 |
Rwork | 0.199 |
R-free | 0.24210 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1wz2 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX (1.14_3260) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 2.540 |
High resolution limit [Å] | 2.490 | 6.780 | 2.500 |
Rmerge | 0.088 | 0.035 | 0.704 |
Rmeas | 0.094 | 0.037 | 0.767 |
Rpim | 0.030 | 0.012 | 0.297 |
Number of reflections | 100132 | 5378 | 4772 |
<I/σ(I)> | 6.6 | ||
Completeness [%] | 99.9 | 99.9 | 98.6 |
Redundancy | 9.5 | 9.4 | 6.2 |
CC(1/2) | 0.997 | 0.723 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | EVAPORATION | 289 | 20% (w/v) PEG 6000, 100 mM Tris-cl, pH 8.0, and 200 mM Lithium chloride |