6LOJ

The complex structure of IpaH9.8-LRR and hGBP1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U1
Synchrotron site	SSRF
Beamline	BL17U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-05-09
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.97
Spacegroup name	P 61 2 2
Unit cell lengths	122.490, 122.490, 582.350
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	60.910 - 3.720
R-factor	0.2287
Rwork	0.227
R-free	0.25550
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1f5n
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHENIX
Refinement software	PHENIX (1.17.1_3660)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	60.910	3.853
High resolution limit [Å]	3.720	3.720
Number of reflections	28447	2754
<I/σ(I)>	12.52	2.59
Completeness [%]	99.7	99.38
Redundancy	37.3	12.1
CC(1/2)	0.996	0.775

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6	298	2.5M NaCl, 0.1M K/Na phosphate buffer, pH 6