6LOI
Crystal structure of Enterococcus faecalis Undecaprenyl pyrophosphate synthase(EfaUPPS)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U1 |
| Synchrotron site | SSRF |
| Beamline | BL17U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-06-21 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.97853 |
| Spacegroup name | P 31 |
| Unit cell lengths | 82.779, 82.779, 213.706 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 35.351 - 2.503 |
| R-factor | 0.1803 |
| Rwork | 0.178 |
| R-free | 0.23820 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ueh |
| Data reduction software | HKL-3000 |
| Data scaling software | Aimless |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.14_3260) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.540 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.472 | |
| Rmeas | 0.539 | |
| Rpim | 0.269 | |
| Number of reflections | 54407 | 1926 |
| <I/σ(I)> | 1.2 | 1.2 |
| Completeness [%] | 96.5 | 69 |
| Redundancy | 6.6 | 3 |
| CC(1/2) | 0.993 | 0.778 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 0.1 M citrate acid/sodium hydroxide (pH 5.0), 20% (w/v) PEG 6000 |
