6LLB

Crystal structure of mpy-RNase J (mutant S247A), an archaeal RNase J from Methanolobus psychrophilus R15, in complex with 6 nt RNA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	298
Detector technology	PIXEL
Collection date	2018-09-16
Detector	PSI PILATUS 6M
Wavelength(s)	0.9785
Spacegroup name	P 65 2 2
Unit cell lengths	168.726, 168.726, 166.427
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	46.746 - 2.600
R-factor	0.1897
Rwork	0.188
R-free	0.22430
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5ws2
Data reduction software	XDS
Data scaling software	Aimless (0.7.3)
Phasing software	PHASER
Refinement software	PHENIX (1.14_3260)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	48.710	48.710	2.700
High resolution limit [Å]	2.600	9.730	2.600
Rmerge	0.082	0.018	0.938
Rmeas	0.085	0.019	0.969
Rpim	0.021	0.005	0.242
Total number of observations	671046	11913	70100
Number of reflections	43478	968	4459
<I/σ(I)>	31.7	105.7	4.2
Completeness [%]	100.0	99.2	100
Redundancy	15.4	12.3	15.7
CC(1/2)	1.000	1.000	0.933

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	298	0.01 M MgSO4, 0.05 M (CH3)2AsO2Na (pH 6.5) and 2 M (NH4)2SO4.