6LJN

Crystal structure of human Sirt5 in complex with the fluorogenic tetrapeptide substrate P15

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-01-03
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	0.97890
Spacegroup name	P 21 2 21
Unit cell lengths	42.100, 55.403, 124.685
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	41.410 - 1.800
R-factor	0.175
Rwork	0.173
R-free	0.21200
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5xhs
RMSD bond length	0.010
RMSD bond angle	1.230
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	PHASER
Refinement software	PHENIX (1.10.1_2155)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.840
High resolution limit [Å]	1.796	1.800
Number of reflections	28011
<I/σ(I)>	17.01
Completeness [%]	99.9
Redundancy	12.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		289	23%-27% (v/v) PEG 3350, 0.1 M MES, pH 5.5-6.0, 0.1-0.2 M NaCl