6LIV

Crystal structure of Tyrosine decarboxylase in complex with PLP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-1A
Synchrotron site	Photon Factory
Beamline	BL-1A
Temperature [K]	80
Detector technology	PIXEL
Collection date	2018-12-15
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1.10000
Spacegroup name	P 21 2 21
Unit cell lengths	117.911, 180.414, 218.057
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.840 - 2.310
R-factor	0.2277
Rwork	0.227
R-free	0.24990
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1js3
RMSD bond length	0.005
RMSD bond angle	0.673
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX (1.16_3549)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.450
High resolution limit [Å]	2.310	2.310
Rmeas	0.214	0.887
Number of reflections		31999
<I/σ(I)>	7.12	1.7
Completeness [%]	99.7	98.4
Redundancy	6.96	6.66
CC(1/2)	0.991	0.712

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	disodium malonate, HEPES, TCEP-HCl, glycerol