6LIS

ASFV dUTPase in complex with dUMP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	CCD
Collection date	2019-04-11
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.97853
Spacegroup name	P 21 21 21
Unit cell lengths	96.179, 103.060, 102.885
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	30.608 - 1.998
R-factor	0.1885
Rwork	0.187
R-free	0.22600
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2hqu
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.14_3260)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.610	2.070
High resolution limit [Å]	1.998	2.000
Rmerge		0.687
Rmeas		0.709
Rpim		0.200
Number of reflections	69859	6889
<I/σ(I)>	2.68	3.5
Completeness [%]	100.0	100
Redundancy	15	1.24
CC(1/2)	0.994	0.883

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	0.2 M sodium citrate, 0.1 m, 24% (w/v) PEG 3350