6LES
3D domain-swapped dimer of the maltose-binding protein fused to a fragment of the focal adhesion kinase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 2 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-07-13 |
| Detector | DECTRIS EIGER X 9M |
| Wavelength(s) | 0.980 |
| Spacegroup name | P 1 |
| Unit cell lengths | 65.910, 71.960, 83.390 |
| Unit cell angles | 97.84, 90.03, 106.55 |
Refinement procedure
| Resolution | 47.450 - 2.004 |
| R-factor | 0.2015 |
| Rwork | 0.200 |
| R-free | 0.23050 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5bmy |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0253) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.490 | 2.076 |
| High resolution limit [Å] | 2.000 | 2.004 |
| Rmerge | 0.103 | 0.944 |
| Rmeas | 0.123 | |
| Rpim | 0.066 | 0.723 |
| Number of reflections | 93102 | 8178 |
| <I/σ(I)> | 8.07 | 1.2 |
| Completeness [%] | 95.3 | 84.49 |
| Redundancy | 8.12 | 1.2 |
| CC(1/2) | 0.995 | 0.600 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 10.5 | 298 | 2000mM Ammonium sulfate, 100mM CAPS/ Sodium hydroxide pH 10.5 |
