6LDC
Structure of Bifidobacterium dentium beta-glucuronidase complexed with C6-nonyl uronic isofagomine
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSRRC BEAMLINE BL13C1 |
| Synchrotron site | NSRRC |
| Beamline | BL13C1 |
| Temperature [K] | 110 |
| Detector technology | CCD |
| Collection date | 2013-08-01 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 92.659, 105.144, 160.967 |
| Unit cell angles | 90.00, 91.09, 90.00 |
Refinement procedure
| Resolution | 28.056 - 2.181 |
| R-factor | 0.1503 |
| Rwork | 0.150 |
| R-free | 0.17660 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3k46 |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.107 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.8.2_1309) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 30.000 | 30.000 | 2.260 |
| High resolution limit [Å] | 2.180 | 4.690 | 2.180 |
| Rmerge | 0.050 | 0.027 | 0.168 |
| Total number of observations | 738778 | ||
| Number of reflections | 160237 | 16291 | 15991 |
| <I/σ(I)> | 15.2 | ||
| Completeness [%] | 99.9 | 99.8 | 99.9 |
| Redundancy | 4.6 | 4.5 | 4.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 298 | 0.1 M sodium cacodylate, pH 6.5, 8% w/v PEG 20K |
