6LBM

Crystal Structure of FOXC2-DBD bound to a palindromic DNA sequence

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U1
Synchrotron site	SSRF
Beamline	BL17U1
Temperature [K]	100
Detector technology	CCD
Collection date	2017-11-05
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.97915
Spacegroup name	C 1 2 1
Unit cell lengths	96.521, 42.656, 103.277
Unit cell angles	90.00, 103.16, 90.00

Refinement procedure

Resolution	39.281 - 2.841
R-factor	0.234
Rwork	0.233
R-free	0.26260
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6ako
RMSD bond length	0.005
RMSD bond angle	0.709
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.11.1_2575)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	39.281	2.940
High resolution limit [Å]	2.840	2.840
Number of reflections	9399	547
<I/σ(I)>	15.43	1.8
Completeness [%]	94.6
Redundancy	7.1
CC(1/2)	0.986	0.718

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		277	50 mM HEPES, pH 7.5, 8%-12% PEG4000 (w/v), 250 mM NaCl, 10 mM MgCl2 and 1 mM TCEP.