6LBI

Crystal Structure of FOXO1-DBD homodimer bound to a palindromic DNA sequence

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U1
Synchrotron site	SSRF
Beamline	BL17U1
Temperature [K]	100
Detector technology	CCD
Collection date	2016-06-25
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.97915
Spacegroup name	P 21 21 21
Unit cell lengths	67.732, 89.090, 194.166
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	46.793 - 3.067
R-factor	0.2286
Rwork	0.227
R-free	0.26410
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3co6
RMSD bond length	0.003
RMSD bond angle	0.467
Data reduction software	HKL-2000 (3.25)
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.11.1_2575)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	46.793	3.170
High resolution limit [Å]	3.060	3.060
Rpim	0.107	1.208
Number of reflections	17379	314
<I/σ(I)>	8.8
Completeness [%]	99.0
Redundancy	10.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		277	50 mM NaAc, pH 4.7, 12%-18% PEG4000 (w/v), 200 mM NaCl, 10 mM MgCl2 and 1 mM TCEP.