6LB8

Crystal structure of the Ca2+-free T4L-MICU1-MICU2 complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-03-07
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	0.97892
Spacegroup name	P 21 21 21
Unit cell lengths	79.339, 133.296, 178.023
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.056 - 3.283
R-factor	0.188
Rwork	0.183
R-free	0.24940
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4nsc 6iih
RMSD bond length	0.014
RMSD bond angle	1.294
Data reduction software	HKL-3000 (v705c)
Data scaling software	HKL-3000 (v705c)
Phasing software	PHENIX (1.10.1_2155)
Refinement software	PHENIX (1.10.1_2155)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.060	3.401
High resolution limit [Å]	3.283	3.283
Rmerge	0.195	0.505
Rmeas	0.203	0.525
Number of reflections	29560	2680
<I/σ(I)>	15.12
Completeness [%]	99.0	100
Redundancy	13	12.9
CC(1/2)	0.958	0.937

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5	293.15	2% tacsimate pH 5.0, 0.1 M sodium citrate pH 5.0, 12% (w/v) PEG 3350