6L9W

Crystal structure of mouse TIFA (T9E/C36S mutant)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL41XU
Synchrotron site	SPring-8
Beamline	BL41XU
Temperature [K]	100
Detector technology	CCD
Collection date	2013-01-30
Detector	RAYONIX MX225HE
Wavelength(s)	1
Spacegroup name	C 1 2 1
Unit cell lengths	149.278, 115.885, 80.938
Unit cell angles	90.00, 106.77, 90.00

Refinement procedure

Resolution	37.290 - 2.900
R-factor	0.2155
Rwork	0.213
R-free	0.26640
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6l9u
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	MOLREP
Refinement software	PHENIX (1.13_2998)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	37.290	3.000
High resolution limit [Å]	2.900	2.900
Rmerge	0.084	0.294
Number of reflections	28175	2170
<I/σ(I)>	23.3
Completeness [%]	95.7
Redundancy	3.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.4	293	MES pH 6.4, MPD