6L9J
Structure of yeast Snf5 and Swi3 subcomplex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL19U1 |
| Synchrotron site | SSRF |
| Beamline | BL19U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-06-14 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.9789 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 205.522, 146.459, 154.329 |
| Unit cell angles | 90.00, 131.20, 90.00 |
Refinement procedure
| Resolution | 48.621 - 2.642 |
| R-factor | 0.231 |
| Rwork | 0.229 |
| R-free | 0.27950 |
| Structure solution method | SAD |
| RMSD bond length | 0.009 |
| RMSD bond angle | 0.889 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX (1.10.1_2155) |
| Refinement software | PHENIX (1.10.1_2155) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.700 |
| High resolution limit [Å] | 2.640 | 2.650 |
| Rpim | 0.076 | 0.365 |
| Number of reflections | 99168 | 4909 |
| <I/σ(I)> | 8.79 | |
| Completeness [%] | 99.3 | |
| Redundancy | 4.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 277 | 100mM HEPES (pH 7.8), 230mM Li2SO4, 20%-24% (w/v) PEG 3350 |
