6L9C
Neutron structure of copper amine oxidase from Arthrobacter glibiformis at pD 7.4
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE BL-5A |
Synchrotron site | Photon Factory |
Beamline | BL-5A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2016-05-31 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 1.0 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 157.548, 61.779, 92.332 |
Unit cell angles | 90.00, 112.13, 90.00 |
Refinement procedure
Resolution | 20.951 - 1.720 |
R-factor | 0.1815 |
Rwork | 0.180 |
R-free | 0.20950 |
RMSD bond length | 0.009 |
RMSD bond angle | 1.318 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX (1.11.1_2575) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 1.160 |
High resolution limit [Å] | 1.140 | 3.090 | 1.140 |
Rmerge | 0.069 | 0.056 | 0.801 |
Rmeas | 0.076 | 0.062 | 0.950 |
Rpim | 0.029 | 0.027 | 0.504 |
Number of reflections | 296297 | 13805 | 14901 |
<I/σ(I)> | 14.8 | ||
Completeness [%] | 99.4 | 90.6 | 99.9 |
Redundancy | 5.2 | 6.3 | 3.5 |
CC(1/2) | 0.992 | 0.703 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | MICRODIALYSIS | 7.4 | 289 | 1.05M potassium-sodium tartrate, 25mM HEPES |
1 | MICRODIALYSIS | 7.4 | 289 | 1.05M potassium-sodium tartrate, 25mM HEPES |