6L84
Complex of DNA polymerase IV and D-DNA duplex
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PAL/PLS BEAMLINE 5C (4A) |
Synchrotron site | PAL/PLS |
Beamline | 5C (4A) |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2015-07-08 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.9794 |
Spacegroup name | P 2 21 21 |
Unit cell lengths | 52.763, 98.664, 100.769 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 44.872 - 2.602 |
R-factor | 0.1869 |
Rwork | 0.182 |
R-free | 0.22630 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2bq3 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHENIX |
Refinement software | PHENIX (1.13_2998) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 2.640 |
High resolution limit [Å] | 2.600 | 7.050 | 2.600 |
Rmerge | 0.122 | 0.118 | 0.359 |
Rmeas | 0.127 | 0.123 | 0.384 |
Rpim | 0.036 | 0.036 | 0.133 |
Number of reflections | 26904 | 932 | 835 |
<I/σ(I)> | 11.7 | ||
Completeness [%] | 99.8 | 99 | 99.8 |
Redundancy | 11.3 | 12.4 | 7.7 |
CC(1/2) | 0.981 | 0.858 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 292 | 0.1 M BIS-TRIS (pH 7.0), 0.1 M calcium acetate, 2% glycerol, and 9% w/v PEG3350 |