6L65

Sirtuin 2 protein with H3K18 myristoylated peptide

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U
Synchrotron site	SSRF
Beamline	BL17U
Temperature [K]	100
Detector technology	CCD
Collection date	2018-05-14
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.97906
Spacegroup name	P 1 21 1
Unit cell lengths	36.062, 73.399, 55.693
Unit cell angles	90.00, 93.86, 90.00

Refinement procedure

Resolution	55.594 - 1.800
R-factor	0.1789
Rwork	0.177
R-free	0.21840
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4r8m
RMSD bond length	0.007
RMSD bond angle	0.955
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	PHASER
Refinement software	PHENIX (1.12_2829)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	55.600	1.660
High resolution limit [Å]	1.580	1.580
Number of reflections	29350	1169
<I/σ(I)>	11.7
Completeness [%]	74.0
Redundancy	6.06
CC(1/2)	0.992	0.522

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	EVAPORATION	6.5	293.1	0.1M MES, pH 6.5, 6%-20% (w/v) PEG 10000