6L5N

Crystal structure of human DEAD-box RNA helicase DDX21 at post-unwound state

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-01-11
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.97849
Spacegroup name	P 1 21 1
Unit cell lengths	72.360, 89.230, 79.690
Unit cell angles	90.00, 115.60, 90.00

Refinement procedure

Resolution	33.520 - 2.242
R-factor	0.1781
Rwork	0.175
R-free	0.23300
Structure solution method	SAD
Data reduction software	HKL-2000
Data scaling software	SCALA (3.3.22)
Phasing software	PHASER
Refinement software	PHENIX (1.14_3260)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	71.867	39.505	2.360
High resolution limit [Å]	2.242	7.090	2.242
Rmerge		0.065	0.449
Rmeas	0.111	0.085	0.533
Rpim	0.044	0.034	0.207
Total number of observations	276183
Number of reflections	43794	1427	6378
<I/σ(I)>	11.6	7.4	1.6
Completeness [%]	99.7	98.8	99.9
Redundancy	6.3	6.2	6.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	293	7% MPD and 0.1M Bicine, pH8.5