6KYT
The structure of the M. tb toxin MazEF-mt1 complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL19U1 |
| Synchrotron site | SSRF |
| Beamline | BL19U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-07-08 |
| Detector | DECTRIS PILATUS3 S 6M |
| Wavelength(s) | 0.979 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 51.697, 161.140, 78.851 |
| Unit cell angles | 90.00, 99.63, 90.00 |
Refinement procedure
| Resolution | 28.681 - 2.001 |
| R-factor | 0.196306485999 |
| Rwork | 0.195 |
| R-free | 0.22984 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5wyg |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.635 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | MOLREP |
| Refinement software | PHENIX (1.10.1_2155) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.070 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.076 | 0.907 |
| Number of reflections | 85400 | 8535 |
| <I/σ(I)> | 18.1 | 1.3 |
| Completeness [%] | 99.8 | 99.7 |
| Redundancy | 6.8 | 6.6 |
| CC(1/2) | 0.995 | 0.698 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 9 | 293 | 25% PEG1500, 0.1M BIS-Tris pH 9.0, 0.1M NaCl |
