6KVD

Crystal structure of human nucleosome containing H2A.J

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-1A
Synchrotron site	Photon Factory
Beamline	BL-1A
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-11-25
Detector	DECTRIS EIGER X 4M
Wavelength(s)	1.1000
Spacegroup name	P 21 21 21
Unit cell lengths	99.640, 108.791, 171.177
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	47.835 - 2.210
R-factor	0.215
Rwork	0.213
R-free	0.25740
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5y0c
Data reduction software	XDS (Jan 26, 2018 BUILT=20180808)
Data scaling software	XDS (Jan 26, 2018 BUILT=20180808)
Phasing software	PHASER (2.8.2)
Refinement software	PHENIX (1.14_3260)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	47.835	2.340
High resolution limit [Å]	2.210	6.570	2.210
Rmerge	0.176	0.047	2.004
Rmeas	0.183	0.049	2.077
Number of reflections	93881	3824	14912
<I/σ(I)>	13.05	48.94	1.25
Completeness [%]	99.9	99.6	99.4
Redundancy	14	13.165	14.399
CC(1/2)	0.998	0.999	0.520

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6	293	potassium cacodylate, potassium chloride, manganese chloride