6KUY
Crystal structure of the alpha2A adrenergic receptor in complex with a partial agonist
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL41XU |
Synchrotron site | SPring-8 |
Beamline | BL41XU |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2016-04-24 |
Detector | DECTRIS PILATUS3 S 6M |
Wavelength(s) | 0.97 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 71.210, 71.620, 286.250 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 47.620 - 3.200 |
R-factor | 0.2683 |
Rwork | 0.267 |
R-free | 0.29800 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2y02 |
RMSD bond length | 0.003 |
RMSD bond angle | 0.628 |
Data reduction software | XDS |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | PHENIX ((1.13_2998: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 47.700 | 3.400 |
High resolution limit [Å] | 3.200 | 3.200 |
Rmerge | 0.100 | 0.550 |
Number of reflections | 11252 | 11252 |
<I/σ(I)> | 2.1 | |
Completeness [%] | 90.5 | 90.5 |
Redundancy | 10.7 | 6.3 |
CC(1/2) | 0.998 | 0.998 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | LIPIDIC CUBIC PHASE | 293 | 0.1M HEPES sodium pH 7.4, 310mM Ammonium tartrate dibasic, 36.5% PEG400 |