6KRV
Crystal structure of mouse IgG2b Fc complexed with B domain of Protein A
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE AR-NW12A |
| Synchrotron site | Photon Factory |
| Beamline | AR-NW12A |
| Temperature [K] | 90 |
| Detector technology | CCD |
| Collection date | 2014-06-13 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 1.0000 |
| Spacegroup name | P 42 21 2 |
| Unit cell lengths | 145.280, 145.280, 155.528 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.980 - 3.300 |
| R-factor | 0.2102 |
| Rwork | 0.208 |
| R-free | 0.25030 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2rgs |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.317 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | MOLREP |
| Refinement software | PHENIX (1.11.1_2575) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.530 |
| High resolution limit [Å] | 3.300 | 3.300 |
| Rmerge | 0.080 | 0.884 |
| Number of reflections | 25631 | 4547 |
| <I/σ(I)> | 26.1 | 3.8 |
| Completeness [%] | 99.9 | 99.9 |
| Redundancy | 14.4 | 14.6 |
| CC(1/2) | 0.999 | 0.936 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | 60% (v/v) Tacsimate (pH 7.0) |
