6KR6

Crystal structure of Drosophila Piwi

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL32XU
Synchrotron site	SPring-8
Beamline	BL32XU
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-02-15
Detector	DECTRIS EIGER X 9M
Wavelength(s)	1.0000
Spacegroup name	P 21 21 21
Unit cell lengths	62.090, 115.640, 119.890
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	39.900 - 2.900
R-factor	0.2404
Rwork	0.239
R-free	0.25850
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5guh
RMSD bond length	0.002
RMSD bond angle	0.566
Data reduction software	DIALS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.16_3549)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	39.930	39.930	3.080
High resolution limit [Å]	2.900	8.520	2.900
Rmerge	0.548	0.194	21.100
Rmeas	0.551	0.196	21.241
Number of reflections	36974	1445	5991
<I/σ(I)>	10.12	44.58	0.52
Completeness [%]	100.0	99	100
Redundancy	74.447	79.409	75.512
CC(1/2)	0.998	0.998	0.676

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6	293	20 % PEG 6000, 0.1 M MES pH6.0, and 0.01 M zinc chloride