6KR4

Crystal structure of the liprin-alpha3_SAM123/LAR_D1D2 complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-11-05
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.98
Spacegroup name	C 1 2 1
Unit cell lengths	250.378, 147.678, 143.972
Unit cell angles	90.00, 103.85, 90.00

Refinement procedure

Resolution	42.173 - 2.850
R-factor	0.1922
Rwork	0.192
R-free	0.22980
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1lar 3tac
RMSD bond length	0.007
RMSD bond angle	0.922
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.900
High resolution limit [Å]	2.850	7.730	2.850
Rmerge	0.150	0.089	1.068
Rmeas	0.160	0.095	1.137
Rpim	0.055	0.032	0.383
Number of reflections	118298	6010	5872
<I/σ(I)>	3.8
Completeness [%]	99.7	98.9	99.9
Redundancy	8.2	8	8.6
CC(1/2)		0.992	0.744

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	296	50mM Tris-HCl, 200mM NaCl, 1mM ethylenediaminetetraacetic acid, 1mM dithiothreitol