6KR1

ATP dependent protease HslV from Staphylococcus aureus

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 7A (6B, 6C1)
Synchrotron site	PAL/PLS
Beamline	7A (6B, 6C1)
Temperature [K]	100
Detector technology	CCD
Collection date	2018-03-28
Detector	ADSC QUANTUM 270
Wavelength(s)	0.97933
Spacegroup name	P 32
Unit cell lengths	94.304, 94.304, 227.374
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	33.160 - 2.000
R-factor	0.2187
Rwork	0.216
R-free	0.26590
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1yyf
RMSD bond length	0.001
RMSD bond angle	0.378
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Refinement software	PHENIX (1.16_3549)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.030
High resolution limit [Å]	2.000	2.000
Rmeas	0.082	0.526
Rpim	0.028	0.260
Number of reflections	150435	7367
<I/σ(I)>	17.6	2.2
Completeness [%]	98.6	96.6
Redundancy	6.5	3.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	287	HEPES:NaOH, Lithium Sulfate