6KQP
NSD1 SET domain in complex with SAM
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 21-ID-F |
Synchrotron site | APS |
Beamline | 21-ID-F |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2014-10-07 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 0.97872 |
Spacegroup name | P 42 21 2 |
Unit cell lengths | 91.649, 91.649, 60.582 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 45.870 - 2.400 |
R-factor | 0.1993 |
Rwork | 0.194 |
R-free | 0.24660 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3ooi |
RMSD bond length | 0.008 |
RMSD bond angle | 1.474 |
Data reduction software | DENZO |
Data scaling software | HKL-2000 |
Phasing software | MOLREP |
Refinement software | REFMAC (5.8.0258) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.440 |
High resolution limit [Å] | 2.400 | 2.400 |
Number of reflections | 10515 | 465 |
<I/σ(I)> | 30 | 2.35 |
Completeness [%] | 99.5 | |
Redundancy | 16.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 294 | 100mM sodium citrate, pH 5.6, 200mM potassium sodium tartrate, 2M ammonium sulfate |