6KO7
Crystal structure of the Ethidium bound RamR determined with XtaLAB Synergy
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU FR-X |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2016-09-15 |
Detector | RIGAKU HyPic-6000HE |
Wavelength(s) | 1.5406 |
Spacegroup name | P 1 |
Unit cell lengths | 44.164, 54.276, 91.412 |
Unit cell angles | 103.42, 97.45, 90.14 |
Refinement procedure
Resolution | 23.450 - 1.700 |
R-factor | 0.1718 |
Rwork | 0.169 |
R-free | 0.23480 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3vvy |
RMSD bond length | 0.013 |
RMSD bond angle | 1.845 |
Data reduction software | CrysalisPro |
Data scaling software | Aimless (0.5.29) |
Phasing software | MOLREP |
Refinement software | REFMAC (5.8.0238) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 23.450 | 1.730 |
High resolution limit [Å] | 1.700 | 1.700 |
Rmerge | 0.069 | 0.402 |
Rmeas | 0.084 | 0.548 |
Rpim | 0.047 | 0.371 |
Number of reflections | 88793 | 4303 |
<I/σ(I)> | 7.2 | |
Completeness [%] | 98.7 | 94.8 |
Redundancy | 2.4 | 1.8 |
CC(1/2) | 0.994 | 0.479 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 5.6 | 298 | PEG3350, ammonium sulfate, sodium Citrate |