6KMV
caspase-11 C254A P22/P10 in complex with mouse GSDMD-C domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NFPSS BEAMLINE BL19U1 |
| Synchrotron site | NFPSS |
| Beamline | BL19U1 |
| Temperature [K] | 93 |
| Detector technology | PIXEL |
| Collection date | 2018-12-23 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.97852 |
| Spacegroup name | P 1 |
| Unit cell lengths | 89.798, 139.207, 175.942 |
| Unit cell angles | 92.43, 99.06, 96.35 |
Refinement procedure
| Resolution | 37.442 - 3.350 |
| R-factor | 0.2194 |
| Rwork | 0.219 |
| R-free | 0.25200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6kmu |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.070 | 3.430 |
| High resolution limit [Å] | 3.350 | 3.350 |
| Rmerge | 0.093 | 0.577 |
| Number of reflections | 118363 | 8903 |
| <I/σ(I)> | 11.1 | 2.23 |
| Completeness [%] | 98.3 | |
| Redundancy | 3.41 | |
| CC(1/2) | 0.996 | 0.771 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 200 mM sodium malonate (pH 6.5), 18% polyethylene glycol 3350, and 10 mM TCEP |
