6KK1

Structure of thermal-stabilised(M8) human GLP-1 receptor transmembrane domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL41XU
Synchrotron site	SPring-8
Beamline	BL41XU
Temperature [K]	80
Detector technology	CCD
Collection date	2017-01-17
Detector	RAYONIX MX225HE
Wavelength(s)	1.0
Spacegroup name	P 1
Unit cell lengths	64.930, 67.350, 83.680
Unit cell angles	91.07, 90.10, 107.96

Refinement procedure

Resolution	49.504 - 2.800
R-factor	0.2458
Rwork	0.244
R-free	0.29000
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5vew
RMSD bond length	0.009
RMSD bond angle	1.441
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX ((1.14_3260: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.500	2.950
High resolution limit [Å]	2.800	2.800
Rmerge	0.130	0.400
Number of reflections	25859	3446
<I/σ(I)>	5.3	2.2
Completeness [%]	77.9	70.9
Redundancy	3.1	2.6
CC(1/2)	0.980	0.760

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	LIPIDIC CUBIC PHASE		293	0.4-0.45 M ammonium acetate, 0.1 M sodium cacodylate, pH 6.2-6.6, 35-38% PEG400, 3% w/v aminohexanoic acid