6KJ7
E. coli ATCase catalytic subunit mutant - G166P
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU R-AXIS IV |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2016-07-27 |
| Detector | RIGAKU RAXIS IV++ |
| Wavelength(s) | 1.54 |
| Spacegroup name | H 3 |
| Unit cell lengths | 128.923, 128.923, 48.253 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 44.293 - 2.839 |
| R-factor | 0.2148 |
| Rwork | 0.210 |
| R-free | 0.25970 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1za1 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.14_3260) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.890 |
| High resolution limit [Å] | 2.839 | 2.840 |
| Number of reflections | 7077 | 338 |
| <I/σ(I)> | 31.6 | |
| Completeness [%] | 99.5 | 94.9 |
| Redundancy | 3.3 | |
| CC(1/2) | 1.000 | 0.820 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 0.2M NH4Ac, 0.1 M Tris pH 8.5, 20% PEG3350, and 10% glycerol |
